In cell culture, cell culture bottle is often used in a container, it uses a wide neck design, easy to operate. The growth of cells requires specific conditions, of which the sterile environment is very important.

Nontoxicity and sterility are the primary conditions for cultured cells in vitro. In vivo, the detoxification system and immune system can resist the invasion of microorganisms or other harmful substances, but in the process of cell culture in vitro, such as cell culture bottle, the lack of immune system protection and loss of microbial defense ability and detoxification ability to harmful substances. To ensure that cells can grow and reproduce in the external environment, it is necessary to ensure a sterile work area, good personal hygiene, sterile reagents and media, and sterile handling.

Of course, aseptic environment is only one of the important conditions for cell culture in the cell culture bottle. Others include the right temperature, osmotic pressure, and PH of the gas environment, which are the basic conditions required for cell growth.

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